LifestyleArtsEntertainmentFilmNewsLocal NewsMayor congratulates Limerick’s Ruth Negga on Oscar nominationBy Editor – January 24, 2017 1098 Walk in Covid testing available in Limerick from Saturday 10th April No vaccines in Limerick yet Print Shannondoc operating but only by appointment RELATED ARTICLESMORE FROM AUTHOR WhatsApp Linkedin Twitter Facebook Catalyst International Film Festival launches today Email First Irish death from Coronavirus Previous articleMunster confirm Hart & Farrell capture as Hanrahan returnsNext articleGuitar night for Dolan’s Editor Limerick City and County Mayor, Cllr Kieran O’Hanlon has congratulated actor Ruth Negga on her Oscar nomination for her role in the film ‘Loving’.“I was absolutely delighted to hear the news, and all of Limerick is certainly rooting for her,” said Mayor O’Hanlon. “It’s great for Limerick – we’re on the map now in the film industry, especially with our new purpose built Troy Film Studios in Castletroy open for business.”Displaying his knowledge of cinema through the ages, the Mayor pointed to another famous local actor in commending Negga’s achievements, “Ruth is the second Limerick person to be nominated for an Academy Award. Richard Harris was nominated twice.”Sign up for the weekly Limerick Post newsletter Sign Up “Ruth has done her family in Dooradoyle and Limerick very proud,” the Mayor said. “I will be writing to her on behalf of the citizens of Limerick to congratulate her on her nomination and the best of luck at the ceremony next month. Win or lose, I would love to invite her and her family to a Mayoral Reception, when her schedule allows,” he concluded. Advertisement TAGSfeaturedMayor Kieran O’HanlonRuth Negga Surgeries and clinic cancellations extended
Imaging captures how blood stem cells take root Researchers at Boston Children’s Hospital’s Stem Cell Research Program were able, for the first time, to use patients’ own cells to create cells similar to those in bone marrow, and then use them to identify potential treatments for a blood disorder. The work was published today by Science Translational Medicine.The team derived the so-called blood progenitor cells from two patients with Diamond-Blackfan anemia (DBA), a rare, severe blood disorder in which the bone marrow cannot make enough oxygen-carrying red blood cells. The researchers first converted some of the patients’ skin cells into induced pluripotent stem (iPS) cells. They then got the iPS cells to make blood progenitor cells, which they loaded into a high-throughput drug screening system. Testing a library of 1,440 chemicals, the team found several that showed promise in a dish. One compound, SMER28, was able to get live mice and zebrafish to start churning out red blood cells.The study marks an important advance in the stem cell field. Induced pluripotent stem cells, which are theoretically capable of making virtually any cell type, were first created in the lab in 2006 from skin cells treated with genetic reprogramming factors. Specialized cells generated by iPS cells have been used to look for drugs for a variety of diseases — except for blood disorders, because of technical problems in getting iPS cells to make blood cells. Related Step-by-step observations will help scientists enhance bone-marrow transplants Sergei Doulatov, co-first author on the paper with Linda Vo and Elizabeth Macari, said the cells “have been hard to instruct when it comes to making blood. This is the first time iPS cells have been used to identify a drug to treat a blood disorder.”DBA currently is treated with steroids, but these drugs help only about half of patients, and some of them eventually stop responding. When steroids fail, patients must receive lifelong blood transfusions and quality of life for many patients is poor. The researchers believe SMER28 or a similar compound might offer another option.“It is very satisfying as physician scientists to find new potential treatments for rare blood diseases such as Diamond-Blackfan anemia,” said Leonard Zon, director of Boston Children’s Stem Cell Research Program and co-corresponding author on the paper with George Q. Daley, “This work illustrates a wonderful triumph,” said Daley, associate director of the Stem Cell Research Program and also dean of Harvard Medical School.Making red blood cellsAs in DBA itself, the patient-derived blood progenitor cells, studied in a dish, failed to generate the precursors of red blood cells, known as erythroid cells. The same was true when the cells were transplanted into mice. But the chemical screen got several “hits”: In wells loaded with these chemicals, erythroid cells began appearing.Because of its especially strong effect, SMER28 was put through additional testing. When used to treat the marrow in zebrafish and mouse models of DBA, the animals made erythroid progenitor cells that in turn made red blood cells, reversing or stabilizing anemia. The same was true in cells from DBA patients transplanted into mice. The higher the dose of SMER28, the more red blood cells were produced, and no ill effects were found. (Formal toxicity studies have not yet been conducted.)Circumventing a roadblockPrevious researchers have tried for years to isolate blood stem cells from patients. They have sometimes succeeded, but the cells are very rare and cannot create enough copies of themselves to be useful for research. Attempts to get iPS cells to make blood stem cells have also failed.The Boston Children’s researchers were able to circumvent these problems by instead transforming iPS cells into blood progenitor cells using a combination of five reprogramming factors. Blood progenitor cells share many properties with blood stem cells and are readily multiplied in a dish.“Drug screens are usually done in duplicate, in tens of thousands of wells, so you need a lot of cells,” said Doulatov, who now heads a lab at the University of Washington. “Although blood progenitor cells aren’t bona fide stem cells, they are multipotent and they made red cells just fine.”SMER28 has been tested preclinically for some neurodegenerative diseases. It activates a so-called autophagy pathway that recycles damaged cellular components. In DBA, SMER28 appears to turn on autophagy in erythroid progenitors. Doulatov plans to further explore how this interferes with red blood cell production.